PNAS4 Antibody

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ProSci
Product Code: PSI-5217
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5217-0.02mg0.02mg£150.00
Quantity:
PSI-5217-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of PNAS4 in EL4 cell lysate with PNAS4 antibody at (A) 1 and (B) 2 μg/mL.
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Western blot analysis of PNAS4 in EL4 cell lysate with PNAS4 antibody at (A) 1 and (B) 2 μg/mL.

Documents

Further Information

Additional Names:
PNAS4 Antibody: DESI, DESI1, DeSI-2, PNAS-4, PPPDE1, CGI-146, FAM152A, C1orf121, Desumoylating isopeptidase 2, PPPDE peptidase domain-containing protein 1
Application Note:
PNAS4 antibody can be used for detection of PNAS4 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
PNAS4 Antibody: PNAS4 is a highly conserved protein that shares high homology from plants to animals. It was initially identified as a putative apoptosis-related protein in the human acute promyelocytic leukemia cell line NB4. PNAS4 is activated during the early response to DNA damage and can inhibit proliferation via apoptosis when overexpressed in some tumor cells such as U2OS, SKOV3, and A549. PNAS4 inhibits tumor cell proliferation through the following mechanisms: (i) overexpression of PNAS4 causes S phase arrest by regulating the expression of cell cycle-related proteins and (ii) PNAS4 induces apoptosis through the mitochondrial apoptosis pathway. Recent evidence has shown that PNAS4 may be involved in the genesis of some cancers and could be a potential candidate for lung cancer therapy alone or in combination with gemcitabine. At least two isoforms of PNAS4 are known to exist.
Background References:
  • Yao S, Xie L, Qian M, et al. Pnas4 is a novel regulator for convergence and extension during vertebrate gastrulation. FEBS Lett.2008; 582:2325-32.
  • Yan F, Gou L, Yang J, et al. A novel pro-apoptosis gene PNAS4 that induces apoptosis in A549 human lung adenocarcinoma cells and inhibits tumor growth in mice. Biochimie2009; 91:502-7.
  • Yuan Z, Liu H, Yan F, et al. Improved therapeutic efficacy against murine carcinoma by combining honokiol with gene therapy of PNAS-4, a novel pro-apoptotic gene. Cancer Sci.2009; 100:1757-66.
  • Yuan Z, Yan F, Wang YS, et al. PNAS-4, a novel pro-apoptotic gene, can potentiate antineoplastic effects of cisplatin. Cancer Chemother. Pharmacol.2009; .
Buffer:
PNAS4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%)
Immunogen:
PNAS4 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PNAS4.

The immunogen is located within the last 50 amino acids of PNAS4.
NCBI Gene ID #:
51029
NCBI Official Name:
desumoylating isopeptidase 2
NCBI Official Symbol:
DESI2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057160
Protein GI Number:
38708309
Purification:
PNAS4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
Q9BSY9
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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