CDC42 Antibody
| Code | Size | Price |
|---|
| PSI-5101-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5101-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Chicken
Antibody Isotype: IgY
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
CDC42 Antibody: G25K, CDC42Hs, Cell division control protein 42 homolog, G25K GTP-binding protein
Application Note:
CDC42 antibody can be used for detection of CDC42 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
CDC42 Antibody: CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to S. cerevisiae CDC42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. CDC42 is thought to regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. At least two isoforms of CDC42 are known to exist.
Background References:
- Erickson JW and Cerione RA. Multiple roles for Cdc42 in cell regulation. Curr. Opin. Cell Biol.2001; 13:153-7.
- Chen W, Lim HH, and Lim L. The CDC42 homologue from Caenorhabditis elegans. Complementation of yeast mutation. J. Biol. Chem.1993; 268:13280-5.
- Hart MJ, Eva A, Evans T, et al. Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product. Nature1991; 354:311-4.
- Rohatgi R, Ma L, Miki H, et al. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly. Cell1999; 97:221-31.
Buffer:
CDC42 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%), Bovine: (100%), Guinea pig: (100%)
Immunogen:
CDC42 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CDC42.
The immunogen is located within amino acids 40 - 90 of CDC42.
The immunogen is located within amino acids 40 - 90 of CDC42.
NCBI Gene ID #:
998
NCBI Official Name:
cell division cycle 42 (GTP binding protein, 25kDa)
NCBI Official Symbol:
CDC42
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001782
Protein GI Number:
4757952
Purification:
CDC42 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
P60953
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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