KLHL15 Antibody

ProSci
Product Code: PSI-5223
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5223-0.02mg0.02mg£150.00
Quantity:
PSI-5223-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of KLHL15 in HeLa cell lysate with KLHL15 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunocytochemistry of KLHL15 in HeLa cells with KLHL15 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of KLHL15 in HeLa cells with KLHL15 antibody at 20 μg/mL.

Western blot analysis of KLHL15 in HeLa cell lysate with KLHL15 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of KLHL15 in HeLa cells with KLHL15 antibody at 5 μg/mL.
Immunofluorescence of KLHL15 in HeLa cells with KLHL15 antibody at 20 μg/mL.

Further Information

Additional Names:
KLHL15 Antibody: HEL-S-305, KIAA1677, Kelch-like protein 15
Application Note:
KLHL15 antibody can be used for detection of KLHL15 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
KLHL15 Antibody: KLHL15 is a member of a growing superfamily of kelch repeat-containing proteins. The kelch motif was discovered in the sequence of the Drosophila kelch ORF1 protein and forms a conserved beta-propeller tertiary structure. These kelch-like proteins have been implicated in embryogenesis and carcinogenesis through cytoskeleton organization. KLHL15 mRNA is widely expressed in many tissues and is thought to be involved in protein ubiquination and cytoskeleton organization.
Background References:
  • Yoshida K. Identification and characterization of a novel kelch-like gene KLHL15 in silico. Oncol. Rep.2005; 13:1133-7.
  • Robinson DN and Cooley L. Drosophila kelch is an oligomeric ring canal actin organizer. J. Cell Biol.1997; 138:799-810.
  • Adams J, Kelso R, and Cooley L. The kelch repeat superfamily of proteins: propellers of cell function. Trends Cell Biol.2000; 10:17-24.
Buffer:
KLHL15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
KLHL15 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human KLHL15.

The immunogen is located within the last 50 amino acids of KLHL15.
NCBI Gene ID #:
80311
NCBI Official Name:
kelch-like 15 (Drosophila)
NCBI Official Symbol:
KLHL15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_085127
Protein GI Number:
226442729
Purification:
KLHL15 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q96M94
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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