THEM2 Antibody
| Code | Size | Price |
|---|
| PSI-5257-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5257-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
THEM2 Antibody: HT012, THEM2, PNAS-27, HT012, Acyl-coenzyme A thioesterase 13, Thioesterase superfamily member 2, Acyl-CoA thioesterase 13
Application Note:
THEM2 antibody can be used for detection of THEM2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
THEM2 Antibody: THEM2, also known as ACOT13, belongs to the hotdog-fold superfamily and possesses thioesterase activity, with greater activity observed with longer chain acyl-CoAs such as myristoyl- and palmitoyl-CoA. THEM2 is highly expressed in several tissues such as heart, kidney, liver and brain and has been shown to be co-localized with beta-tubulin on microtubules. THEM2 interacts with StarD, a protein that plays a key role in fatty acid metabolism, and the addition of StarD to THEM2 increased its catalytic activity, suggesting that THEM2 plays a significant role in the metabolism of fatty acids. At least two isoforms of THEM2 are known to exist.
Background References:
- Cheng Z, Song F, Shan X, et al. Crystal structure of human thioesterase superfamily member 2. Biochem. Biophys. Res. Commun.2006; 349:172-7.
- Wei J, Kang HW, and Cohen DE. Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs. Biochem. J.2009; 421:311-22.
- Cheng Z, Bao S, Shan X, et al. Human thioesterase superfamily member 2 (hTHEM2) is co-localized with beta-tubulin onto the microtubule. Biochem. Biophys. Res. Commun.2006; 350:850-3.
- Scappa EF, Pocai A, Wu MK, et al. Regulation of energy substrate utilization and hepatic insulin sensitivity by phosphatidylcholine transfer protein/StarD. FASEB J.2008; 22:2579-90.
Buffer:
THEM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
THEM2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human THEM2.
The immunogen is located within the last 50 amino acids of THEM2.
The immunogen is located within the last 50 amino acids of THEM2.
NCBI Gene ID #:
55856
NCBI Official Name:
acyl-CoA thioesterase 13
NCBI Official Symbol:
ACOT13
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060943
Protein GI Number:
8923812
Purification:
THEM2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Obesity
Swissprot #:
Q9NPJ3
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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