LIAR Antibody

ProSci
Product Code: PSI-5261
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5261-0.02mg0.02mg£150.00
Quantity:
PSI-5261-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of LIAR in mouse kidney tissue lysate with LIAR antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 3
Immunohistochemistry of LIAR in mouse kidney tissue with LIAR antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of LIAR in Mouse Kidney cells with LIAR antibody at 20 μg/mL.

Western blot analysis of LIAR in mouse kidney tissue lysate with LIAR antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of LIAR in mouse kidney tissue with LIAR antibody at 2.5 μg/mL.
Immunofluorescence of LIAR in Mouse Kidney cells with LIAR antibody at 20 μg/mL.

Further Information

Additional Names:
LIAR Antibody: LIAR, LIAR, Ankyrin repeat domain-containing protein 54, Lyn-interacting ankyrin repeat protein
Application Note:
LIAR antibody can be used for detection of LIAR by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
LIAR Antibody: Ankyrin (ANK) repeats mediate protein-protein interactions in diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20. ANK repeats may occur in combinations with other types of domains. The structural repeat unit contains two anti-parallel helices and a beta-hairpin, with repeats stacked in a superhelical arrangement. LIAR, also known as ANKRD54, is a recently identified ANK repeat-containing protein that is predominantly expressed in tissues rich in cilliated cells, such as olfactory sensory neurons and is predicted to be important to cilia. At least three isoforms of LIAR are known to exist.
Background References:
  • Li J, Mahajan A, and Tsai MD. Ankyrin repeat: a unique motif mediating protein-protein interactions. Biochemistry2006; 45:15168-78.
Buffer:
LIAR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
LIAR antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human LIAR.

The immunogen is located within amino acids 230 - 280 of LIAR.
NCBI Gene ID #:
129138
NCBI Official Name:
ankyrin repeat domain 54
NCBI Official Symbol:
ANKRD54
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_620152
Protein GI Number:
20270347
Purification:
LIAR Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q6NXT1
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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