MPYS Antibody

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ProSci
Product Code: PSI-5269
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5269-0.02mg0.02mg£150.00
Quantity:
PSI-5269-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: MPYS 5269 (1 μg/mL), MPYS 64-236 (2 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: MPYS 5269 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Human THP-1 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: MPYS 5269 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Western Blot Validation in Mouse A-20 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: MPYS 5269 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 Immunocytochemistry Validation of MPYS in Mouse A20 Cells</strong><br> Immunocytochemical analysis of A20 cells using anti-MPYS antibody (5269) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 6 Immunofluorescence Validation of MPYS in Moue A20 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed A20 Cells labeling MPYS with 5269 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: MPYS 5269 (1 μg/mL), MPYS 64-236 (2 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: MPYS 5269 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human THP-1 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: MPYS 5269 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse A-20 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: MPYS 5269 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunocytochemistry Validation of MPYS in Mouse A20 Cells</strong><br> Immunocytochemical analysis of A20 cells using anti-MPYS antibody (5269) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunofluorescence Validation of MPYS in Moue A20 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed A20 Cells labeling MPYS with 5269 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Documents

Further Information

Additional Names:
MPYS Antibody: ERIS, MITA, MPYS, NET23, STING, ERIS, Stimulator of interferon genes protein, Endoplasmic reticulum interferon stimulator, hSTING
Application Note:
WB: 1-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
MPYS Antibody: MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals. It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling. MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression. It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS. This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription. At least three isoforms of MPYS are known to exist.
Background References:
  • Jin et al. Mol. Cell. Biol.2008; 28:5014-26.
  • Zhong et al. Immunity2008; 29:538-50.
Buffer:
MPYS Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat (82%), Bovine: (82%), Pig: (82%), Chicken: (71%)
Immunogen:
Anti-MPYS antibody (5269) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human MPYS.

The immunogen is located within amino acids 260-310 of MPYS.
ISOFORMS:
Human MPYS has one isoform (379aa, 42kD). Mouse MPYS has 3 isoforms, including isoform 1 (379aa, 42kD), isoform 2 (427aa, 48kD) and isoform 3 (337aa, 38kD). Rat MPYS also has one isoform (379aa, 43kD). 5269 can detect human, mouse and rat.
NCBI Gene ID #:
340061
NCBI Official Name:
transmembrane protein 173
NCBI Official Symbol:
TMEM173
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 42kD

Observed: 42 kD
Protein Accession #:
NP_938023
Protein GI Number:
38093659
Purification:
MPYS Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q86WV6
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar MPYS expression profile in human cell lines detected by two independent anti-MPYS antibodies that recognize different epitopes, 5269 against near C-terminus domain and 64-236 against C-terminus domain.  MPYS proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

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