MAK10 Antibody
| Code | Size | Price |
|---|
| PSI-5273-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5273-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
MAK10 Antibody: EGAP, MAK10, MAK10P, bA379P1.1, EGAP, N-alpha-acetyltransferase 35, NatC auxiliary subunit, Embryonic growth-associated protein homolog
Application Note:
MAK10 antibody can be used for detection of MAK10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
MAK10 Antibody: The MAK10 gene encodes a 733-amino acid protein with several regions of similarity to T cell receptor alpha-subunit V (variable) regions in yeast. The mammalian homologue of yeast MAK10, also known as EGAP, is one subunit of a novel N-terminal acetyltransferase (NAT) that is highly conserved among vertebrate species. It is expressed in a variety of tissues in the developing rat embryo but restricted in expression in the adult, remaining detectable only in tissues undergoing continual cell renewal or in cells responding to pathological injury. The MAK10-NAT complex is an essential regulatory enzyme controlling the function of a subset of proteins required for embryonic growth control and vessel development. This complex functionally co-assembles in mammalian cells to regulate cell proliferation and is essential for embryonic development, at least in part through the regulation of target of rapamycin (TOR) signaling events. At least two isoforms of MAK10 are known to exist.
Background References:
- Lee YJ et al. MAK10, a glucose repressible gene necessary for replication of a dsRNA virus of Saccharomyces cerevisiae, has T cell receptor alpha-subunit motifs. Genetics1992; 132:87-96.
- Yi XJ, et al. A novel epithelial wound-related gene is abundantly expressed in developing rat cornea and skin. Curr. Eye Res.2000; 20:430-40.
- Wenzlau JM, et al. Embryonic growth-associated protein is one subunit of a novel N-terminal acetyltransferase complex essential for embryonic vascular development. Circ. Res.2006; 98:846-55.
- Weiser-Evans MC, et al. Novel embryonic genes are preferentially expressed by autonomously replicating rat embryonic and neointimal smooth muscle cells. Circ. Res.2000; 87:608-15.
Buffer:
MAK10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%)
Immunogen:
MAK10 antibody was raised against a 14 amino acid synthetic peptide near the center of human MAK10.
The immunogen is located within amino acids 420 - 470 of MAK10.
The immunogen is located within amino acids 420 - 470 of MAK10.
NCBI Gene ID #:
60560
NCBI Official Name:
N(alpha)-acetyltransferase 35, NatC auxiliary subunit
NCBI Official Symbol:
MAK10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_078911
Protein GI Number:
145275204
Purification:
MAK10 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
Q5VZE5
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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