SCAMP4 Antibody

ProSci
Product Code: PSI-5289
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5289-0.02mg0.02mg£150.00
Quantity:
PSI-5289-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of SCAMP4 in 3T3 cell lysate with SCAMP4 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of SCAMP4 in human brain tissue with SCAMP4 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of SCAMP4 in Human Brain cells with SCAMP4 antibody at 20 μg/mL.

Western blot analysis of SCAMP4 in 3T3 cell lysate with SCAMP4 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of SCAMP4 in human brain tissue with SCAMP4 antibody at 2.5 μg/mL.
Immunofluorescence of SCAMP4 in Human Brain cells with SCAMP4 antibody at 20 μg/mL.

Further Information

Additional Names:
SCAMP4 Antibody: SCAMP-4, Secretory carrier-associated membrane protein 4, Secretory carrier membrane protein 4
Application Note:
SCAMP4 antibody can be used for detection of SCAMP4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SCAMP4 Antibody: Secretory carrier membrane proteins (SCAMPs) are integral membrane proteins seen in secretory and endocytic carriers that are thought to play roles in membrane trafficking. One member of this family, SCAMP4, lacks the putative calcium binding and leucine zipper structures as well as the protein-protein interacting NPF domains common to most SCAMP family members. In rats, SCAMP4 mRNA is repressed by progesterone in brain regions associated with female sexual behavior. At least three isoforms of SCAMP4 are known to exist. This SCAMP4 antibody is predicted to not cross-react with any other member of the secretory carrier membrane protein family.
Background References:
  • Hubbard C, Singleton D, Rauch M, et al. The secretory carrier membrane protein family: structure and membrane topology. Mol. Biol. Cell2000; 11:2933-47.
  • Krebs CJ and Pfaff DW. Expression of the SCAMP-4 gene, a new member of the secretory carrier membrane protein family, is repressed by progesterone in brain regions associated with female sexual behavior. Brain Res. Mol. Brain Res.2001; 88:144-54.
Buffer:
SCAMP4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen:
SCAMP4 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SCAMP4.

The immunogen is located within the last 50 amino acids of SCAMP4.
NCBI Gene ID #:
113178
NCBI Official Name:
secretory carrier membrane protein 4
NCBI Official Symbol:
SCAMP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_524558
Protein GI Number:
17738287
Purification:
SCAMP4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q969E2
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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