NOTUM Antibody
| Code | Size | Price |
|---|
| PSI-5291-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5291-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
NOTUM Antibody: Protein notum homolog
Application Note:
NOTUM antibody can be used for detection of NOTUM by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
NOTUM Antibody: In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by beta-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.
Background References:
- Giraldez AJ, Copley RR, and Cohen SM. HSPG modification by the secreted enzyme Notum shapes the wingless morphogen gradient. Dev. Cell2002; 667-76.
- Traister A, Shi W, and Filmus J. Mammalian Notum induces the release of glypicans and other GPI-anchored proteins from the cell surface. Biochem. J.2008; 410:503-11.
- Torisu Y, Watanabe A, Nonaka A, et al. Human homolog of NOTUM, overexpressed in hepatocellular carcinoma, is regulated transcriptionally by b-catenin/TCF. Cancer Sci.2008; 6:1139-46.
Buffer:
NOTUM Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
NOTUM antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NOTUM.
The immunogen is located within amino acids 90 - 140 of NOTUM.
The immunogen is located within amino acids 90 - 140 of NOTUM.
NCBI Gene ID #:
147111
NCBI Official Name:
notum pectinacetylesterase homolog (Drosophila)
NCBI Official Symbol:
NOTUM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_848588
Protein GI Number:
194394139
Purification:
NOTUM Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q6P988
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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