JMJD1A Antibody

Contact us
ProSci
Product Code: PSI-5365
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5365-0.02mg0.02mg£150.00
Quantity:
PSI-5365-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 5
<strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: JMJD1A, 5365 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
2 / 5
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: JMJD1A, 5365 (2 μg/mL), JMJD1A, 5367 (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
3 / 5
<strong>Figure 3 Western Blot Validation in Human HepG2 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: JMJD1A, 5365 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
4 / 5
<strong>Figure 4 Immunohistochemistry Validation of JMJD1A in Rat Liver Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-JMJD1A antibody (5365) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
5 / 5
<strong>Figure 5 Immunofluorescence Validation of JMJD1A in Rat Liver Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat liver cells labeling JMJD1A with 5365 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
❮❯

<strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: JMJD1A, 5365 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: JMJD1A, 5365 (2 μg/mL), JMJD1A, 5367 (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human HepG2 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: JMJD1A, 5365 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of JMJD1A in Rat Liver Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-JMJD1A antibody (5365) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunofluorescence Validation of JMJD1A in Rat Liver Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat liver cells labeling JMJD1A with 5365 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Documents

Further Information

Additional Names:
JMJD1A Antibody: TSGA, JMJD1, JHDM2A, JHMD2A, JMJD1A, KIAA0742, TSGA, Lysine-specific demethylase 3A, JmjC domain-containing histone demethylation protein 2A
Application Note:
WB: 1-2 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples; Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
JMJD1A Antibody: The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist.
Background References:
  • Hoog et al. Mol. Reprod. Dev.1991; 30:173-81.
  • Sar et al. Cell Tissue Res.2009; 337:223-34.
  • Yamane et al. Cell. 2006; 125:483-95.
  • Knebel et al. J. Cell Biochem.2006; 99:319-29.
Buffer:
JMJD1A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (94%), Chicken: (87%)
Immunogen:
Anti-JMJD1A antibody (5365) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human JMJD1A.

The immunogen is located within amino acids 50-100 of JMJD1A.
ISOFORMS:
Human JMJD1A has one isoform (1321aa, 147kD). Mouse JMJD1A has 2 isoforms, including isoform 1 (1323aa, 148kD) and isoform 2 (831aa, 94kD). Rat JMJD1A has one isoform (1323aa, 148kD). 5365 can detect human, mouse and rat.
NCBI Gene ID #:
55818
NCBI Official Name:
lysine (K)-specific demethylase 3A
NCBI Official Symbol:
KDM3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 147kD

Observed: 147kD
Protein Accession #:
NP_060903
Protein GI Number:
20357522
Purification:
JMJD1A Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
This antibody will not cross-react with JMJD1B or JMJD1C.
Swissprot #:
Q9Y4C1
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar JMJD1A expression profile in human cell lines detected by two independent anti-JMJD1A antibodies that recognize different epitopes, 5365 against N-terminus domain and 5367 against central domain.  JMJD1A proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Related Products

Product NameProduct CodeSupplier 
JMJD1A PeptidePSI-5365PProSci Summary Details