JMJD4 Antibody

ProSci
Product Code: PSI-5379
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5379-0.02mg0.02mg£150.00
Quantity:
PSI-5379-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of JMJD4 in human spleen tissue lysate with JMJD4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 3
Immunohistochemistry of JMJD4 in rat spleen tissue with JMJD4 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of JMJD4 in rat spleen tissue with JMJD4 antibody at 5 μg/mL.

Western blot analysis of JMJD4 in human spleen tissue lysate with JMJD4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of JMJD4 in rat spleen tissue with JMJD4 antibody at 2.5 μg/mL.
Immunofluorescence of JMJD4 in rat spleen tissue with JMJD4 antibody at 5 μg/mL.

Further Information

Additional Names:
JMJD4 Antibody: JmjC domain-containing protein 4, Jumonji domain-containing protein 4
Application Note:
JMJD4 antibody can be used for detection of JMJD4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
JMJD4 Antibody: The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. While several JMJD proteins have been identified as being involved in chromatin regulation, histone demethylation and development, the JMJD4 has not been identified. JMJD4 is expressed in multiple tissues and At least three isoforms of JMJD4 are known to exist.
Background References:
  • Takeuchi T, Watanabe Y, Takano-Shimizu T, et al. Roles of jumonji and jumonji family genes in chromatin regulation and development. Dev. Dyn.2006; 235:2449-59.
Buffer:
JMJD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (71%)
Immunogen:
JMJD4 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human JMJD4.

The immunogen is located within amino acids 130 - 180 of JMJD4.
NCBI Gene ID #:
65094
NCBI Official Name:
jumonji domain containing 4
NCBI Official Symbol:
JMJD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_075383
Protein GI Number:
239788847
Purification:
JMJD4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
This antibody will not recognize other members of the JMJD family.
Swissprot #:
Q9H9V9
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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