SKA1 Antibody
| Code | Size | Price |
|---|
| PSI-5401-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5401-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SKA1 Antibody: C18orf24, C18orf24, Spindle and kinetochore-associated protein 1
Application Note:
SKA1 antibody can be used for detection of SKA1 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
SKA1 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing.
Background References:
- Cleveland DW, Mao Y, and Sullivan KF. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling. Cell2003; 112:407-21.
- Hanisch A, Sillje HHW, and Nigg EA. Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska EMBO J.2006; 25:5504-15.
- Gaitanos TN, Santamaria A, Jeyaprakash AA, et al. Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf EMBO J.2009; 28:1442-52.
Buffer:
SKA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (85%)
Immunogen:
SKA1 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA1.
The immunogen is located within amino acids 180 - 230 of SKA1.
The immunogen is located within amino acids 180 - 230 of SKA1.
NCBI Gene ID #:
220134
NCBI Official Name:
spindle and kinetochore associated complex subunit 1
NCBI Official Symbol:
SKA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q96BD8
Protein GI Number:
74731226
Purification:
SKA1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q96BD8
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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