SKA2 Antibody

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ProSci
Product Code: PSI-5403
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5403-0.02mg0.02mg£150.00
Quantity:
PSI-5403-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of SKA2 in 3T3 cell lysate with SKA2 antibody at (A) 0.5 and (B) 1 μg/mL.
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Western blot analysis of SKA2 in 3T3 cell lysate with SKA2 antibody at (A) 0.5 and (B) 1 μg/mL.

Documents

Further Information

Additional Names:
SKA2 Antibody: FAM33A, FAM33A, Spindle and kinetochore-associated protein 2, Protein FAM33A
Application Note:
SKA2 antibody can be used for detection of SKA2 by Western blot at 0.5 - 1 μg/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
SKA2 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.
Background References:
  • Cleveland DW, Mao Y, and Sullivan KF. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling. Cell2003; 112:407-21.
  • Hanisch A, Sillje HHW, and Nigg EA. Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska EMBO J.2006; 25:5504-15.
  • Gaitanos TN, Santamaria A, Jeyaprakash AA, et al. Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf EMBO J.2009; 28:1442-52.
  • Rice L, Water CE, Eccles J, et al. Identification and functional analysis of SKA2 interaction with the glucocorticoid receptor. J. Endocrinol.2008; 198:499-509.
Buffer:
SKA2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%), Rat: (80%)
Immunogen:
SKA2 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human SKA2.

The immunogen is located within the first 50 amino acids of SKA2.
NCBI Gene ID #:
348235
NCBI Official Name:
spindle and kinetochore associated complex subunit 2
NCBI Official Symbol:
SKA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_872426
Protein GI Number:
32699054
Purification:
SKA2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q8WVK7
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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