SKA3 Antibody
| Code | Size | Price |
|---|
| PSI-5405-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5405-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SKA3 Antibody: RAMA1, C13orf3, RAMA1, Spindle and kinetochore-associated protein 3
Application Note:
SKA3 antibody can be used for detection of SKA3 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
SKA3 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.
Background References:
- Cleveland DW, Mao Y, and Sullivan KF. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling. Cell2003; 112:407-21.
- Hanisch A, Sillje HHW, and Nigg EA. Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska EMBO J.2006; 25:5504-15.
- Gaitanos TN, Santamaria A, Jeyaprakash AA, et al. Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf EMBO J.2009; 28:1442-52.
- Daum JR, Wren JD, Daniel JJ, et al. Ska3 is required for spindle checkpoint silencing and maintenance of chromosome cohesion in mitosis. Curr. Biol.2009; 19:1467-72.
Buffer:
SKA3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SKA3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA3.
The immunogen is located within amino acids 290 - 340 of SKA3.
The immunogen is located within amino acids 290 - 340 of SKA3.
NCBI Gene ID #:
221150
NCBI Official Name:
spindle and kinetochore associated complex subunit 3
NCBI Official Symbol:
SKA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_659498
Protein GI Number:
260763910
Purification:
SKA3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q8IX90
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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