RWDD3 Antibody
| Code | Size | Price |
|---|
| PSI-5409-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5409-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
RWDD3 Antibody: RSUME, RSUME, RWD domain-containing protein 3, RWD-containing sumoylation enhancer
Application Note:
RWDD3 antibody can be used for detection of RWDD3 by Western blot at 0.5 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
RWDD3 Antibody: RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune-inflammatory signals. RWDD3 increases IκBeta sumoylation and stability. In addition, RWDD3 inhibits TNF-α-induced kappaB-LUC (Luciferase) reporter activity, showing the functional consequence of IκBeta increased stability. RSUME-enhanced sumoylation of IκBeta leads to the inhibition of NF-κBeta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.
Background References:
- Carbia-Nagashima A, Gerez J, Perez-Castro C, et al. RSUME, a small RWD-containing protein, enhances SUMO conjugation and stabilizes HIF-1alpha during hypoxia. Cell2007; 131:309-23.
- Liberman AC, Druker J, Garcia FA, et al. Intracellular molecular signaling. Basis for specificity to glucocorticoid anti-inflammatory actions. Ann. NY Acad. Sci.2009; 1153:6-13.
- Giacomini D, Haedo M, Gerez J, et al. Differential gene expression in models of pituitary prolactin-producing tumoral cells. Horm. Res.2009; 71 Suppl 2:88-94.
Buffer:
RWDD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (81%)
Immunogen:
RWDD3 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human RWDD3.
The immunogen is located within the first 50 amino acids of RWDD3.
The immunogen is located within the first 50 amino acids of RWDD3.
NCBI Gene ID #:
25950
NCBI Official Name:
RWD domain containing 3
NCBI Official Symbol:
RWDD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056300
Protein GI Number:
153252154
Purification:
RWDD3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q9Y3V2
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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