CENPO Antibody
| Code | Size | Price |
|---|
| PSI-5433-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5433-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
CENPO Antibody: CENP-O, MCM21R, ICEN-36, ICEN36, Centromere protein O, Interphase centromere complex protein 36, CENP-O
Application Note:
CENPO antibody can be used for detection of CENPO by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
CENPO Antibody: Accurate chromosome segregation during mitosis requires a kinetochore to assemble correctly at the centromere of each chromatid and form a dynamic interface with the microtubules of the mitotic spindle. The kinetochore assembly includes the multisubunit CENP-H/I complex which can be divided into three functional classes based on phenotype analysis. One of these classes is referred to as the CENP-O class of proteins of which CENPO is the exemplar. These proteins form a stable complex with each other and are required for proper kinetochore function and for recovery from mitotic spindle damage. CENPO has also been shown to be an auto-antigen in a small population of anti-centromere antibodies- (ACA-) positive patients with sclerodoma.
Background References:
- Fukagawa T. Assembly of kinetochore in vertebrate cells. Exp. Cell Res.2004; 296:21-7.
- Okada M, Cheeseman IM, Hori T, et al. The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. Nat. Cell Biol.2006; 8:446-57.
- Hori T, Okada M, Maenaka K, et al. CENP-O class proteins form a stable complex and are required for proper kinetochore function. Mol. Biol. Cell2008; 19:843-54.
- Saito A, Muro Y, Sugiura K, et al. CENP-O, a protein localized at the centromere throughout the cell cycle, is a novel target antigen in systemic sclerosis. J. Rheumatol.2009; 36:781-6.
Buffer:
CENPO Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
CENPO antibody was raised against a 16 amino acid synthetic peptide from near the center of human CENPO.
The immunogen is located within amino acids 170 - 220 of CENPO.
The immunogen is located within amino acids 170 - 220 of CENPO.
NCBI Gene ID #:
79172
NCBI Official Name:
centromere protein O
NCBI Official Symbol:
CENPO
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_077298
Protein GI Number:
13236565
Purification:
CENPO Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q9BU64
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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