GOLPH1 Antibody

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ProSci
Product Code: PSI-5439
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5439-0.02mg0.02mg£150.00
Quantity:
PSI-5439-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of GOLPH1 in K562 cell lysate with GOLPH1 antibody at (A) 1 and (B) 2 μg/mL.
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Western blot analysis of GOLPH1 in K562 cell lysate with GOLPH1 antibody at (A) 1 and (B) 2 μg/mL.

Documents

Further Information

Additional Names:
GOLPH1 Antibody: PAP7, GCP60, GOCAP1, GOLPH1, Golgi resident protein GCP60, Acyl-CoA-binding domain-containing protein 3
Application Note:
GOLPH1 antibody can be used for detection of GOLPH1 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
GOLPH1 Antibody: GOLPH1, also known as GCP60, was initially identified as a Golgi protein that can interact with the integral membrane protein giantin and is thought to be involved in the maintenance of the Golgi structure. GOLPH1 has also been shown to interact with other Golgi proteins such as Golgin-160, a Golgi protein that can be cleaved by caspases-2, -3, and -7, leading to the nuclear localization of Golgin-160. GOLPH1 interaction with the Golgin-160 fragments is stronger than that with the intact Golgin-160, with its interaction regulated by the oxidation state of Cys-463 within GOLPH1, suggesting that the nuclear localization of the caspase-cleaved Golgin-160 fragments is a highly coordinated event. GOLPH1 has also been found to interact with Numb, a cytosolic signaling protein that mediates asymetric cell division of neural progenitor cells to a daughter progenitor cell and a neuron, suggesting that Golgi fragmentation and reconstitution during the cell cycle differentially regulate Numb signaling through changes in GOLPH1 subcellular distribution and may couple cell fate with cell cycle progression.
Background References:
  • Sohda M, Misumi Y, Yamamoto A, et al. Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin. J. Biol. Chem.2001; 276:45298-306.
  • Mancini M, Machamer CE, Roy S, et al. Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis. J. Cell Biol.2000; 149:603-12.
  • Sbodio JI and Machamer CE. Identification of a redox-sensitive cysteine in GCP60 that regulates its interaction with Golgin-160. J. Biol. Chem.2007; 282:29874-81.
  • Zhou Y, Atkins JB, Rompani SB, et al. The mammalian Golgi regulates numb signaling in asymetric cell division by releasing ACBD3 during mitosis. Cell2007; 129:163-78.
Buffer:
GOLPH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (83%), Rat: (83%)
Immunogen:
GOLPH1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human GOLPH1.

The immunogen is located within amino acids 50 - 100 of GOLPH1.
NCBI Gene ID #:
64746
NCBI Official Name:
acyl-Coenzyme A binding domain containing 3
NCBI Official Symbol:
ACBD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
EAW69775
Protein GI Number:
119590181
Purification:
GOLPH1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
Q9H3P7
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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