GOLPH3 Antibody
| Code | Size | Price |
|---|
| PSI-5443-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5443-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
GOLPH3 Antibody: GOPP1, GPP34, MIDAS, Golgi phosphoprotein 3, Coat protein GPP34
Application Note:
GOLPH3 antibody can be used for detection of GOLPH3 by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
GOLPH3 Antibody: GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.
Background References:
- Bell AW, Ward MA, Blackstock WP, et al. Proteomics characterization of abundant Golgi membrane proteins. J. Biol. Chem.2001; 276:5152-65.
- Nakashima-Kamimura N, Asoh S, Ishibashi Y, et al. MIDAS/GPP34, a nuclear gene product, regulates total mitochondrial mass in response to mitochondrial dysfunction. J. Cell Sci.2005; 118:5357-67.
- Scott KL, Kabbarah O, Liang M-C, et al. GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer. Nature2009; 459:1085-90.
Buffer:
GOLPH3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GOLPH3 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human GOLPH3.
The immunogen is located within the first 50 amino acids of GOLPH3.
The immunogen is located within the first 50 amino acids of GOLPH3.
NCBI Gene ID #:
64083
NCBI Official Name:
golgi phosphoprotein 3 (coat-protein)
NCBI Official Symbol:
GOLPH3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH12123
Protein GI Number:
15082415
Purification:
GOLPH3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
Swissprot #:
Q9H4A6
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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