Mimitin Antibody

Contact us
ProSci
Product Code: PSI-5445
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5445-0.02mg0.02mg£150.00
Quantity:
PSI-5445-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

1 / 2
Western blot analysis of Mimitin in Raji cell lysate with Mimitin antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunofluorescence of Mimitin in Raji cells with Mimitin antibody at 20 μg/mL.
❮❯

Western blot analysis of Mimitin in Raji cell lysate with Mimitin antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of Mimitin in Raji cells with Mimitin antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Mimitin Antibody: MMTN, B17.2L, mimitin, NDUFA12L, Mimitin, mitochondrial, Myc-induced mitochondrial protein, MMTN
Application Note:
Mimitin antibody can be used for detection of Mimitin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Mimitin Antibody: Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-κB-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.
Background References:
  • Tsuneoka M, Teye K, Arima M, et al. A novel Myc-target gene, mimitin, that is involved in cell proliferation of esophageal squamous cell carcinoma. J. Biol. Chem.2005; 280:19977-85.
  • Wegrzyn P, Yarwood SJ, Fiegler N, et al. Mimitin - a novel cytokine-regulated mitochondrial protein. BMC Cell Biol.2009; 10:23.
  • Ogilvie I, Kennaway NG, and Shoubridge EA. A molecular chaperone for mitochondrial complex I assembly is mutated in a progressive encephalopathy. J. Clin. Invest.2005; 115:2784-92.
Buffer:
Mimitin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Mimitin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Mimitin.

The immunogen is located within the last 50 amino acids of Mimitin.
NCBI Gene ID #:
91942
NCBI Official Name:
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2
NCBI Official Symbol:
NDUFAF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_777549
Protein GI Number:
29789409
Purification:
Mimitin Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
Swissprot #:
Q8N183
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
Mimitin PeptidePSI-5445PProSci Summary Details