FRMPD1 Antibody

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ProSci
Product Code: PSI-5453
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5453-0.02mg0.02mg£150.00
Quantity:
PSI-5453-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of FRMPD1 in K562 cell lysate with FRMPD1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of FRMPD1 in K562 cells with FRMPD1 antibody at 20 μg/mL.
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Western blot analysis of FRMPD1 in K562 cell lysate with FRMPD1 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of FRMPD1 in K562 cells with FRMPD1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
FRMPD1 Antibody: FRMD2, FRMD2, KIAA0967, FERM and PDZ domain-containing protein 1, FERM domain-containing protein 2
Application Note:
FRMPD1 antibody can be used for detection of FRMPD1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
FRMPD1 Antibody: The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD1 was initially identified through a yeast two-hybrid screening with Activator of G-protein signaling 3 (AGS3), a protein that acts to stabilize the GDP-bound conformation of Gai, as the bait. FRMPD1 binds to the tetratricopeptide repeat of AGS3 and stabilizes AGS3 in a membrane environment. Suppression of FRMPD1 in Cath.a-differentiated neuronal cells decreased the level of endogenous AGS3 in membrane fractions by ~50% and enhanced the inhibition of forskolin-induced increases in cAMP. Co-immunoprecipitation studies indicate that the interaction of AGS3 with FRMPD1 is mutually exclusive with the interaction of AGS3 with Gai3, suggesting that FRMPD1 may position AGS3 in the membrane where it can then interact with Galphai.
Background References:
  • An N, Blumer JB, Bernard ML. The PDZ and Band 4.1 containing protein Frmpd1 regulates the subcellular location of activator of G-protein signaling 3 and its interaction with G-proteins. J. Biol. Chem.2008; 283:24718-28.
  • Stenzel N, Fetzer CP, Heumann R, et al. PDZ-domain-directed basolateral targeting of the peripheral membrane protein FRMPD2 in epithelial cells. J. Cell Sci.2009; 122:3374-84.
  • Lee HW, Choi J, Shin H, et al. Preso, a novel PSD-95-interacting FERM and PDZ domain protein that regulates dendritic spine morphogenesis. J. Neurosci.2008; 28:14546-56.
Buffer:
FRMPD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (93%)
Immunogen:
FRMPD1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human FRMPD1.

The immunogen is located within amino acids 1330 - 1380 of FRMPD1.
NCBI Gene ID #:
22844
NCBI Official Name:
FERM and PDZ domain containing 1
NCBI Official Symbol:
FRMPD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055722
Protein GI Number:
239582741
Purification:
FRMPD1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q5SYB0
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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