FRMPD2 Antibody

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ProSci
Product Code: PSI-5455
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5455-0.02mg0.02mg£150.00
Quantity:
PSI-5455-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of FRMPD2 in rat kidney tissue lysate with FRMPD2 antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunohistochemistry of FRMPD2 in mouse kidney tissue with FRMPD2 antibody at 5 μg/mL.
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Western blot analysis of FRMPD2 in rat kidney tissue lysate with FRMPD2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of FRMPD2 in mouse kidney tissue with FRMPD2 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
FRMPD2 Antibody: PDZK4, PDZD5C, PDZK5C, PDZK4, FERM and PDZ domain-containing protein 2, PDZ domain-containing protein 4
Application Note:
FRMPD2 antibody can be used for detection of FRMPD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
FRMPD2 Antibody: The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD2 also contains an N-terminal KIND domain and three PDZ domains and is structurally similar to the protein tyrosine phosphatase PTP-BL. FRMPD2 is localized in a polarized fashion in epithelial cells at the basolateral membrane and partially co-localizes with the tight-junction marker protein Zonula-occuldens-1. Suppression of FRMPD2 expression via RNAi in Caco-2 cells results in an impairment of tight junction formation, indicating that FRMPD2 plays a major role in tight junction formation. Other experiments indicate that FRMPD2 is a binding partner to several catenin family members and recruitment of FRMPD2 to cell-cell contacts is dependent on E-cadherin-mediated cell-cell adhesion.
Background References:
  • An N, Blumer JB, Bernard ML. The PDZ and Band 4.1 containing protein Frmpd1 regulates the subcellular location of activator of G-protein signaling 3 and its interaction with G-proteins. J. Biol. Chem.2008; 283:24718-28.
  • Stenzel N, Fetzer CP, Heumann R, et al. PDZ-domain-directed basolateral targeting of the peripheral membrane protein FRMPD2 in epithelial cells. J. Cell Sci.2009; 122:3374-84.
  • Lee HW, Choi J, Shin H, et al. Preso, a novel PSD-95-interacting FERM and PDZ domain protein that regulates dendritic spine morphogenesis. J. Neurosci.2008; 28:14546-56.
  • Erdmann KS. The protein tyrosine phosphatase PTP-Basophil/Basophil-like. Interacting proteins and molecular functions. Eur. J. Biochem.270:4789-98.
Buffer:
FRMPD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FRMPD2 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FRMPD2.

The immunogen is located within amino acids 80 - 130 of FRMPD2.
NCBI Gene ID #:
143162
NCBI Official Name:
FERM and PDZ domain containing 2
NCBI Official Symbol:
FRMPD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001018081
Protein GI Number:
281604114
Purification:
FRMPD2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q68DX3
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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