SQSTM1 Antibody
Code | Size | Price |
---|
PSI-5449-0.02mg | 0.02mg | £150.00 |
Quantity:
PSI-5449-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Images
Documents
Further Information
Additional Names:
SQSTM1 Antibody: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, ORCA, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP
Application Note:
WB: 0.5-2 μg/mL; IF: 20 μg/mL; IHC: 2-5 μg/mL;.
Antibody validated: Western Blot in human and mouse samples; Immunofluorescence and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human and mouse samples; Immunofluorescence and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Background:
SQSTM1 Antibody: SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-κB by TNF-α, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1's UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget's disease of bone since the UBA is necessary for aggregate sequestration and cell survival.
Background References:
- Seibenhener et al. Mol. Cell. Biol.2004; 24:8055-68.
- Hocking et al. Hum. Mol. Genet.2002; 11:2735-9.
- Rousiere et al. Best Pract. Res. Clin. Rheumatol.2003; 17:1019-41.
- Layfield et al. Biochem. Soc. Trans.2004; 32:728-30.
Buffer:
SQSTM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SQSTM1 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SQSTM1.
The immunogen is located within the last 50 amino acids of SQSTM1.
The immunogen is located within the last 50 amino acids of SQSTM1.
ISOFORMS:
Human SQSTM1 has two isoforms, including isoform 1 (440aa, 47.7kD) and isoform 2 (356aa, 38.6kD). Mouse SQSTM1 also has two isoforms, including isoform 1 (442aa, 48.2kD) and isoform 2 (404aa, 44.2kD). Rat SQSTM1 has three isoforms, including isoform 1 (439aa, 47.7kD), isoform 2 (412aa, 45kD) and isoform 3 (234aa, 26.1kD). 5449 can detect the isoforms of human mouse and rat except the shortest rat isoform 3.
NCBI Gene ID #:
8878
NCBI Official Name:
sequestosome 1
NCBI Official Symbol:
SQSTM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 48kD
Observed: 65 kD
Observed: 65 kD
Protein Accession #:
Q13501
Protein GI Number:
74735628
Purification:
SQSTM1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q13501
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
KO Validation (Figure 1): Anti-SQSTM1 antibodies (5449) specificity was further verified by SQSTM1 specific knockout. SQSTM1 signal was not detected in SQSTM1 knockout HEK293T cells as compared to that in control wild type cells.
Induced Expression Validation (Figure 2): Raw 264.7 cells were treated with LPS (0.3 µg /mL) for different time period (0-24 hrs). SQSTM1 expression detected by anit-SQSTM1 antibodies (5449) was induced by LPS treatment and the expression levels were increased gradually over the period of 24 hrs.
Related Products
Product Name | Product Code | Supplier | SQSTM1 Peptide | PSI-5449P | ProSci | Summary Details | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|