TMEM16B Antibody
| Code | Size | Price |
|---|
| PSI-5421-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5421-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
TMEM16B Antibody: C12orf3, TMEM16B, C12orf3, Anoctamin-2, Transmembrane protein 16B
Application Note:
TMEM16B antibody can be used for detection of TMEM16B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TMEM16B Antibody: Calcium-activated chloride channels (CaCC) are present in many cell types and mediate physiological functions such as epithelial secretion, sensory signal transduction, and smooth muscle contraction. Subunits of these CaCC's include the transmembrane proteins TMEM16A and TMEM16B. TMEM16B is predicted to have eight transmembrane domains with both the amino and carboxy termini in the cytoplasm and is expressed in several tissues including olfactory sensory neurons as well as photoreceptors in mammalian retina. Like TMEM16A, TMEM16B is thought to form at least part of CaCC's but has different biophysical characteristics such as voltage dependence and unitary conductance.
Background References:
- Schroeder BC, Cheng T, Jan YN, et al. Expression cloning of TMEM16A as a calcium-activated chloride channel subunit. Cell2008; 134:1019-29.
- Stephan AB, Shum EY, Hirsh S, et al. ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification. Proc. Natl. Acad. Sci.USA 2009; 106:11776-81.
- Pifferi S, Dibattista M, and Menini A. TMEM16 induces chloride currents activated by calcium in mammalian cells. Pflugers Arch.2009; 458:1023-38.
- Stohr H, Heisig JB, Benz PM, et al. TMEM16B, a novel protein with calcium-dependent chloride channel activity, associates with a presynaptic protein complex in photoreceptor terminals. J. Neurosci.2009; 29:6809-18.
Buffer:
TMEM16B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TMEM16B antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human TMEM16B.
The immunogen is located within the first 50 amino acids of TMEM16B.
The immunogen is located within the first 50 amino acids of TMEM16B.
NCBI Gene ID #:
57101
NCBI Official Name:
anoctamin 2
NCBI Official Symbol:
ANO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 110 kDa
Observed: 107 kDa
Observed: 107 kDa
Protein Accession #:
NP_001265525
Protein GI Number:
209862785
Purification:
TMEM16B Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q9NQ90
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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