SIPA1L3 Antibody

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ProSci
Product Code: PSI-5495
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5495-0.02mg0.02mg£150.00
Quantity:
PSI-5495-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of SIPA1L3 in SK-N-SH lysate with SIPA1L3 antibody at 1 μg/mL.
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Immunohistochemistry of SIPA1L3 in human brain tissue with SIPA1L3 antibody at 5 μg/mL.
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Immunofluorescence of SIPA1L3 in Human Brain cells with SIPA1L3 antibody at 20 μg/mL.
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Western blot analysis of SIPA1L3 in SK-N-SH lysate with SIPA1L3 antibody at 1 μg/mL.
Immunohistochemistry of SIPA1L3 in human brain tissue with SIPA1L3 antibody at 5 μg/mL.
Immunofluorescence of SIPA1L3 in Human Brain cells with SIPA1L3 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
SIPA1L3 Antibody: SPAL3, KIAA0545, SPAL3, Signal-induced proliferation-associated 1-like protein 3, SPA-1-like protein 3, SIPA1-like protein 3
Application Note:
SIPA1L3 antibody can be used for detection of SIPA1L3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SIPA1L3 Antibody: Signal-induced proliferation associated-like protein 2 (SIPA1L3) is a member of the SIPA1 family of RapGAPs. SIPA1L3 was cloned as a novel molecular component of tight junctions and adherens junctions, suggesting that may function to balance Rap1 by counteracting RapGEFs. Recent studies of SIPA indicate that its deregulation can cause myeloproliferative stem cell disorders in mice and increased metastases in human cancers. Other studies suggest SIPA1L1 may play important roles in embryo development and control of cell proliferation. Based on the amount of homology between SIPA family members, it is possible that SIPA1L3 plays other roles development and cell proliferation.
Background References:
  • Minato N and Hattori M. SPA-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci.2009; 100:17-23.
  • Matsuda M, Kobayashi Y, Masuda S, et al. Identification of adherences junction-associated GTPase activating proteins by the fluorescence localization-based expression cloning. Exp. Cell Res.2008; 314:939-49.
  • Ishida D, Kometani K, Yang H, et al. Myeloproliferative stem cell disorders by deregulated Rap1 activation in SPA-1-deficient mice. Cancer Cell2003; 4:55-65.
  • Park YG, Zhao X, Lesueur F, et al. Sipa1 is a candidate for underlying the metastasis efficiency modifier locus, Mtes. Nat. Genet.2005; 37:1055-62.
Buffer:
SIPA1L3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SIPA1L3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human SIPA1L3.

The immunogen is located within amino acids 80 - 130 of SIPA1L3.
NCBI Gene ID #:
23094
NCBI Official Name:
signal-induced proliferation-associated 1 like 3
NCBI Official Symbol:
SIPA1L3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055888
Protein GI Number:
71143119
Purification:
SIPA1L3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
Swissprot #:
O60292
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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