RHBDD1 Antibody
| Code | Size | Price |
|---|
| PSI-5525-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5525-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
RHBDD1 Antibody: RRP4, RHBDL4, HSD-50, HSD50, Rhomboid-related protein 4, Rhomboid domain-containing protein 1, RRP4
Application Note:
RHBDD1 antibody can be used for detection of RHBDD1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
Background:
RHBDD1 Antibody: The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD1 is highly expressed in the testis and is involved in the cleavage of BIK, a proapoptotic member of the Bcl-2 family. Overexpression or suppression by RNAi of RHBDD1 in 293 cells will reduce or enhance BIK-mediated apoptosis, respectively, demonstrating that RHBDD1 modulates BIK-mediated apoptotic activity. In GC-1 cells, a spermatogonia cell line that can differentiate into spermatids within the seminiferous tubules, suppression of RHBDD1 expression by RNAi caused the cells to lose the ability to survive and differentiate in mouse seminiferous tubules, suggesting that RHBDD1 may be associated with mammalian spermatogenesis.
Background References:
- Koonin EV, Makarova KS, Rogozin IB, et al. The rhomboids: a nearly ubiquitous family of intramembrane serine proteases that probably evolved by multiple horizontal gene transfers. Genome Biol.2003; 4:R19.
- Wang Y, Guan X, Fok KL, et al. A novel member of the rhomboid family, RHBDD1, regulates BIK-mediated apoptosis. Cell Mol. Life Sci.2008; 65:3822-9.
- Wang Y, Song W, Li S, et al. GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules. BNC Cell Biol.2009; 10:25.
Buffer:
RHBDD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (86%)
Immunogen:
RHBDD1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human RHBDD1.
The immunogen is located within the last 50 amino acids of RHBDD1.
The immunogen is located within the last 50 amino acids of RHBDD1.
NCBI Gene ID #:
84236
NCBI Official Name:
rhomboid domain containing 1
NCBI Official Symbol:
RHBDD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAI01265
Protein GI Number:
71680537
Purification:
RHBDD1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
Q8TEB9
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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