Girdin Antibody

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ProSci
Product Code: PSI-5545
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5545-0.02mg0.02mg£150.00
Quantity:
PSI-5545-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of Girdin in rat brain tissue lysate with Girdin antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunofluorescence of Girdin in human brain tissue with Girdin antibody at 20 μg/mL.
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Western blot analysis of Girdin in rat brain tissue lysate with Girdin antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of Girdin in human brain tissue with Girdin antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Girdin Antibody: APE, GIV, GRDN, HkRP1, GIRDIN, KIAA1212, APE, Girdin, Akt phosphorylation enhancer
Application Note:
Girdin antibody can be used for detection of Girdin by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Girdin Antibody: Girdin is a Galpha-interacting protein that can enhance the activation of the protein kinase Akt, remodel the actin cytoskeleton, and is thought to be involved in the regulation of cell migration and cancer metastasis. It has recently been shown that Girdin interacts with Disrupted-in-Schizophrenia 1 (DISC1), a susceptibility gene for major psychiatric disorders. DISC1 is thought to be involved in the migration, positioning and differentiation of dentate granule cells (DGCs) during development; depletion of Girdin or blocking the Girdin-DISC1 interaction results in defects in axonal sprouting in the CA3 region of the hippocampus and overextended migration of mispositioning of DGCs, suggesting the Girdin plays a role in postnatal neurogenesis in the dentate gyrus.
Background References:
  • Le-Niculescu H, Niesman I, Fischer T, et al. Identification and characterization of GIV, a novel Galpha i/s-interacting protein found on COPI, endoplasmic reticulum-Golgi transport vesicles. J. Biol. Chem.2005; 280:22012-20.
  • Anai M, Shojima N, Katagiri H, et al. A novel protein kinase B (PKB)/Akt-binding protein enhances PKB kinase activity and regulates DNA synthesis. J. Biol. Chem.2005; 280:18525-35.
  • Enomoto A, Murakami H, Asai N, et al. Akt/PKB regulated actin organization and cell motility via Girdin/APE. Dev. Cell2005; 9:389-402.
  • Enomoto A, Asai N, Namba T, et al. Roles of disrupted-in-schizophrenia 1-interacting protein girdin in postnatal development of the dentate gyrus. Neuron2009; 63:774-87.
Buffer:
Girdin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Girdin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus human Girdin.

The immunogen is located within the last 50 amino acids of Girdin.
NCBI Gene ID #:
55704
NCBI Official Name:
coiled-coil domain containing 88A
NCBI Official Symbol:
CCDC88A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060554
Protein GI Number:
50897852
Purification:
Girdin Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q3V6T2
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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