MRE11 Antibody

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ProSci
Product Code: PSI-5547
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5547-0.02mg0.02mg£150.00
Quantity:
PSI-5547-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of MRE11 in A549 cell lysate with MRE11 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of MRE11 in rat lung tissue with MRE11 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of MRE11 in rat lung tissue with MRE11 antibody at 20 μg/mL.
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Western blot analysis of MRE11 in A549 cell lysate with MRE11 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of MRE11 in rat lung tissue with MRE11 antibody at 5 μg/mL.
Immunofluorescence of MRE11 in rat lung tissue with MRE11 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
MRE11 Antibody: ATLD, HNGS1, MRE11, MRE11B, Double-strand break repair protein MRE11A, Meiotic recombination 11 homolog 1, MRE11 homolog 1
Application Note:
MRE11 antibody can be used for detection of MRE11 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
MRE11 Antibody: MRE11 is involved in the repair of DNA double strand breaks as part of a complex that includes the Rad50 and NBS1 protein and is thought to act in the same pathway as the A-T mutated (ATM) protein. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for non-homologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Mutations in this protein result in a novel ataxia telangiectasia-like disorder (ATLD). Unlike the ATM protein, MRE11 is necessary proper mammalian development.
Background References:
  • Stewart GS, Maser RS, Stankovic T, et al. The DNA double-strand break repair gene hMRE11 is mutated in individuals with an ataxia telangiectasia-like disorder. Cell1999; 99:577-87.
  • Buis J, Wu Y, Deng Y, et al. Mre11 nuclease activity has essential roles in DNA repair and genomic stability distinct from ATM activation. Cell2008; 135:85-96.
Buffer:
MRE11 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (93%)
Immunogen:
MRE11 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus human MRE11.

The immunogen is located within amino acids 40 - 90 of MRE11.
NCBI Gene ID #:
4361
NCBI Official Name:
MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
NCBI Official Symbol:
MRE11A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 78 kDa

Observed: 73 kDa
Protein Accession #:
EAW66932
Protein GI Number:
119587336
Purification:
MRE11 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
P49959
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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