TSPAN9 Antibody

ProSci
Product Code: PSI-5559
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5559-0.02mg0.02mg£150.00
Quantity:
PSI-5559-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of TSPAN9 in EL4 cell lysate with TSPAN9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 3
Immunohistochemistry of TSPAN9 in human spleen tissue with TSPAN9 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of TSPAN9 in human spleen tissue with TSPAN9 antibody at 20 μg/mL.

Western blot analysis of TSPAN9 in EL4 cell lysate with TSPAN9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of TSPAN9 in human spleen tissue with TSPAN9 antibody at 2.5 μg/mL.
Immunofluorescence of TSPAN9 in human spleen tissue with TSPAN9 antibody at 20 μg/mL.

Further Information

Additional Names:
TSPAN9 Antibody: NET5, NET-5, PP1057, NET5, Tetraspanin-9, Tetraspan NET-5, Tspan-9
Application Note:
TSPAN9 antibody can be used for detection of TSPAN9 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TSPAN9 Antibody: The tetraspan family protein members are characterized by four predicted transmembrane domains and are thought to be involved in physiological processes such as tissue differentiation, immunological responses, and sperm-egg fusion. TSPAN9 has recently been identified as a platelet tetraspanin and a component of tetraspanin microdomains that include the collagen receptor GPVI (glycoprotein VI) and integrin alpha6beta1 but not the von Willebrand receptor GPIbalpha or the integrins alphaIIbbeta3 or alpha2beta1, suggesting that TSPAN9 may act to regulate platelet function in concert with other tetraspanins and their associated proteins.
Background References:
  • Berditchevski F and Odintsova E. Tetraspanins as regulators of protein trafficking. Traffic2007; 8:89-96.
  • Serru V, Dessen P, Boucheix C, et al. Sequence and expression of seven new tetraspans. Biochim. Biophys. Acta2000; 1478:159-63.
  • Protty MB, Watkins NA, Colombo D, et al. Identification of Tspan9 as a novel platelet tetraspanin and the collagen receptor GPVI as a component of tetraspanin microdomains. Biochem. J.2009; 417:391-400.
Buffer:
TSPAN9 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Sheep: (100%), Pig: (93%)
Immunogen:
TSPAN9 antibody was raised against a 15 amino acid synthetic peptide from near the center of human TSPAN9.

The immunogen is located within amino acids 90 - 140 of TSPAN9.
NCBI Gene ID #:
10867
NCBI Official Name:
tetraspanin 9
NCBI Official Symbol:
TSPAN9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006666
Protein GI Number:
5729941
Purification:
TSPAN9 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
O75954
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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