VLK Antibody
| Code | Size | Price |
|---|
| PSI-5561-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5561-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
VLK Antibody: Vlk, MAd1, Adtk1, ESTM17, Sgk493, X83346, AI115348, AW548124, Vlk, Protein kinase domain-containing protein, cytoplasmic, Protein kinase-like protein SgK493
Application Note:
VLK antibody can be used for detection of VLK by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
VLK Antibody: VLK was identified as a novel protein kinase that was induced after the differentiation of cultured embryonic stem cells into mesendoderm. It has no homologs in invertebrates, but is highly conserved in vertebrate species although it does not belong to any known protein kinase groups. VLK is initially expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, but its expression is later confined to E-cadherin-negative mesenchyme. It is enriched in the Golgi apparatus and is thought to regulate the rate of protein export from the Golgi. Targeted disruption of VLK in mice leads to a defect in lung development and neonatal lethality. It has been suggested that mutations in VLK may be associated with the allergic condition atopy.
Background References:
- Kinoshita M, Era T, Jakt LM, et al. The novel protein kinase Vlk is essential for stromal function of mesenchymal cells. Development2009; 136:2069-79.
- Castro-Giner F, Bustamante M, Ramon Gonzalez J, et al. A pooling-based genome-wide analysis identifies new potential candidate genes for atopy in the European Community Respiratory Health Survey (ECRHS). BMC Med. Genet.2009; 10:128.
Buffer:
VLK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
VLK antibody was raised against a 15 amino acid synthetic peptide from near the center of human VLK.
The immunogen is located within amino acids 320 - 370 of VLK.
The immunogen is located within amino acids 320 - 370 of VLK.
NCBI Gene ID #:
91461
NCBI Official Name:
protein kinase domain containing, cytoplasmic
NCBI Official Symbol:
Pkdcc
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 32, 54 kDa
Observed: 31, 55 kDa?
Observed: 31, 55 kDa?
Protein Accession #:
NP_612379
Protein GI Number:
238636897
Purification:
VLK Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
Q5RJI4
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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