POU5F1 Antibody

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ProSci
Product Code: PSI-5581
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5581-0.02mg0.02mg£150.00
Quantity:
PSI-5581-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of POU5F1 in mouse liver tissue lysate with POU5F1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of POU5F1 in rat liver tissue with POU5F1 antibody at 5 μgg/mL.
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Western blot analysis of POU5F1 in mouse liver tissue lysate with POU5F1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of POU5F1 in rat liver tissue with POU5F1 antibody at 5 μgg/mL.

Documents

Further Information

Additional Names:
POU5F1 Antibody: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4, OCT3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, 41915
Application Note:
POU5F1 antibody can be used for detection of POU5F1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
POU5F1 Antibody: POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
Background References:
  • Scholer HR, Ruppert S, Suzuki N, et al. New type of POU domain in germ line-specific protein Oct-4. Nature1990; 344:435-9.
  • Scholer HR, Dressler GR, Balling R, et al. Oct-4: a germline-specific transcription factor mapping to the mouse t-complex. EMBO J.1990; 9:2185-95.
  • Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells2003; 5:79-88.
  • Takahashi K and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell2006; 1263:663-76.
Buffer:
POU5F1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%)
Immunogen:
POU5F1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human POU5F1.

The immunogen is located within amino acids 260 - 310 of POU5F1.
NCBI Gene ID #:
5460
NCBI Official Name:
POU class 5 homeobox 1
NCBI Official Symbol:
POU5F1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002692
Protein GI Number:
42560248
Purification:
POU5F1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Stem Cell
Swissprot #:
Q01860
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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