SOX2 Antibody
| Code | Size | Price |
|---|
| PSI-5583-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5583-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SOX2 Antibody: ANOP3, MCOPS3, Transcription factor SOX-2
Application Note:
SOX2 antibody can be used for detection of SOX2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
SOX2 Antibody: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. SOX2 is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. The role of SOX2 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing SOX2 and the transcription factors POU5F1, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Other experiments have shown that iPS cells could be generated using expression plasmids expressing POU5F1, SOX2, KlfF4 and c-Myc, eliminating the need for virus introduction.
Background References:
- Hever AM, Williamson KA, and van Heyningen V. Developmental malformations of the eye: the role of PAX6, SOX2 and OTX2. Clin. Genet.2006; 69:459-70.
- Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells2003; 5:79-88.
- Takahashi K and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell2006; 1263:663-76.
- Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science2008; 322:949-53.
Buffer:
SOX2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Sheep: (100%), Chicken: (100%), Horse: (83%), Pig: (100%)
Immunogen:
SOX2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SOX.
The immunogen is located within the first 50 amino acids of SOX2.
The immunogen is located within the first 50 amino acids of SOX2.
NCBI Gene ID #:
6657
NCBI Official Name:
SRY (sex determining region Y)-box 2
NCBI Official Symbol:
SOX2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003097
Protein GI Number:
28195386
Purification:
SOX2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Stem Cell
Swissprot #:
P48431
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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