NANOG Antibody
| Code | Size | Price |
|---|
| PSI-5585-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5585-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
NANOG Antibody: Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog
Application Note:
NANOG antibody can be used for detection of NANOG by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
NANOG Antibody: Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
Background References:
- Chambers I, Colby D, Robertson M, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell2003; 113:643-55.
- Mitsui K, Tokuzowa Y, Itoh H, et al. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell2003; 113:631-42.
- Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells2003; 5:79-88.
- Scholer HR, Ruppert S, Suzuki N, et al. New type of POU domain in germ line-specific protein Oct-2. Nature1990; 344:435-9.
Buffer:
NANOG Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (89%), Chicken: (94%)
Immunogen:
NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG.
The immunogen is located within amino acids 130 - 180 of NANOG.
The immunogen is located within amino acids 130 - 180 of NANOG.
NCBI Gene ID #:
79923
NCBI Official Name:
Nanog homeobox
NCBI Official Symbol:
NANOG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
EAW88651
Protein GI Number:
119609057
Purification:
NANOG Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Stem Cell
Swissprot #:
Q9H9S0
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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