GATA3 Antibody
| Code | Size | Price |
|---|
| PSI-5599-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5599-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
GATA3 Antibody: HDR, HDRS, Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3
Application Note:
GATA3 antibody can be used for detection of GATA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Background:
GATA3 Antibody: GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer.
Background References:
- Ko LJ, Yamamoto M, Leonard MW, et al. Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. Mol. Cell. Biol. 1991; 11:2778-84.
- Ma GT, Roth ME, Groskopf JC, et al. GATA-2 and GATA-3 regulate trophoblast-specific gene expression in vivo. Dev. 1997; 124:907-14.
- Home P, Ray S, Dutta D, et al. GATA3 is selectively expressed in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expression. J. Biol. Chem. 2009; 284:28729-37.
- Strumpf D, Mao CA, Yamanaka Y, et al. CDX2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Dev. 2005; 132:2093-102.
Buffer:
GATA3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (100%), Pig: (72%)
Immunogen:
GATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GATA3.
The immunogen is located within amino acids 340 - 390 of GATA3.
The immunogen is located within amino acids 340 - 390 of GATA3.
NCBI Gene ID #:
2625
NCBI Official Name:
GATA binding protein 3
NCBI Official Symbol:
GATA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 49 kDa
Observed: 49 kDa
Observed: 49 kDa
Protein Accession #:
NP_001002295
Protein GI Number:
50541959
Purification:
GATA3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Stem Cell
Swissprot #:
P23771
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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