KLF4 Antibody

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ProSci
Product Code: PSI-5603
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5603-0.02mg0.02mg£150.00
Quantity:
PSI-5603-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of KLF4 in human liver tissue lysate with KLF4 antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunohistochemistry of KLF4 in human colon tissue with KLF4 antibody at 5 μg/mL.
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Western blot analysis of KLF4 in human liver tissue lysate with KLF4 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of KLF4 in human colon tissue with KLF4 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
KLF4 Antibody: EZF, GKLF, EZF, Krueppel-like factor 4, Epithelial zinc finger protein EZF
Application Note:
KLF4 antibody can be used for detection of KLF4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
KLF4 Antibody: KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.
Background References:
  • Evans PM, Zhang W, Chen X, et al. Kruppel-like factor 4 is acetylated by p300 and regulates gene transcription via modulation of histone acetylation. J. Bio. Chem.2007; 282:33994-4002.
  • Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells2003; 5:79-88.
  • Takahashi K and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell2006; 1263:663-76.
  • Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science2008; 322:949-53.
Buffer:
KLF4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (80%), Bovine: (74%), Chicken: (75%)
Immunogen:
KLF4 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human KLF4.

The immunogen is located within the last 50 amino acids of KLF4.
NCBI Gene ID #:
9314
NCBI Official Name:
Kruppel-like factor 4 (gut)
NCBI Official Symbol:
KLF4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH30811
Protein GI Number:
21410813
Purification:
KLF4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
O43474
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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