TCF3 Antibody
| Code | Size | Price |
|---|
| PSI-5609-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5609-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
TCF3 Antibody: E2A, E47, ITF1, VDIR, TCF-3, bHLHb21, BHLHB21, E2A, Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21
Application Note:
TCF3 antibody can be used for detection of TCF3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
TCF3 Antibody: The TCF3 gene, also called E2A, encodes two basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. These transcription factors are involved in mediating canonical Wnt signaling, which is very important in a diverse array of cellular functions such as stem cell proliferation, self-renewal, activation, fate determination, differentiation and aging and senescence. They bind beta-catenin and can act as transcriptional activators or repressors for Wnt target genes, and have been shown to regulate specific target genes during CNS development downstream of Wnt signaling. TCF3/Lef complexes are also known to play key roles in controlling cell fate lineages in multipotent skin stem cells.
Background References:
- Korinek V, Barker N, Willert K, et al. Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse. Mol. Cell Biol.1998; 18:1248-1256.
- Gribble SL, Kim HS, Bonner J, et al. Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression. Dev. Cell2009; 136:781-9.
- Cole MF, Johnstone SE, Newman JJ, et al. Tcf3 is an integral component of the core regulatory circuitry of embryonic stem cells. Genes Dev.2008;22:746-55.
- Nguyen H, Rendl M and Fuchs E. Tcf3 governs stem cell features and represses cell fate determination in skin. Cell2006; 127:171-83.
Buffer:
TCF3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (86%)
Immunogen:
TCF3 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TCF3.
The immunogen is located within amino acids 150 - 200 of TCF3.
The immunogen is located within amino acids 150 - 200 of TCF3.
NCBI Gene ID #:
6929
NCBI Official Name:
transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
NCBI Official Symbol:
TCF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003191
Protein GI Number:
27777636
Purification:
TCF3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
P15923
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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