ZSCAN4 Antibody
| Code | Size | Price |
|---|
| PSI-5611-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5611-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ZSCAN4 Antibody: ZNF494, ZNF494, Zinc finger and SCAN domain-containing protein 4, Zinc finger protein 494
Application Note:
ZSCAN4 antibody can be used for detection of ZSCAN4 by Western blot. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
ZSCAN4 Antibody: Zytotic genome activation (ZGA) is the first and most critical transition event from the maternal genetic to the embryonic genetic program and the major burst of ZGA occurs only in the late 2-cell stage. ZSCAN4 encodes a SCAN domain and four zinc finger domains and is expressed in late 2-cell embryos and embryonic stem cells. Loss-of-function studies using siRNA shows delays in the progression from the 2-cell to the 4-cell stage and subsequent embryo development. ZSCAN4 plays an important role in the preimplantation development. Also, ZSCAN4 is involved in telomere maintenance and long term genomic stability in ES cells, suggesting that ZSCAN4 may be of critical importance in maintaining pluripotency in replicating stem cells.
Background References:
- Hamatani T, Carter MG, Sharov AA, et al. Dynamics of global gene expression changes during mouse preimplantation development. Dev. Cell 2004; 6:117-131.
- Falco G, Lee SL, Stanghellini I, et al. Zscan4: a novel gene expressed exclusively in late 2-cell embryos and embryonic stem cells. Dev. Biol. 2007; 307:539-50.
- Zalzman M, Falco G, Sharova LV, et al. Zscan4 regulates telomere elongation and genomic stability in ES cells. Nature 2010; 464:858-63.
Buffer:
ZSCAN4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (81%), Chicken: (86%)
Immunogen:
ZSCAN4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZSCAN4.
The immunogen is located within amino acids 370 - 420 of ZSCAN4.
The immunogen is located within amino acids 370 - 420 of ZSCAN4.
NCBI Gene ID #:
201516
NCBI Official Name:
zinc finger and SCAN domain containing 4
NCBI Official Symbol:
ZSCAN4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 48 kDa
Observed: 64 kDa
Observed: 64 kDa
Protein Accession #:
NP_689890.1
Protein GI Number:
22749365
Purification:
ZSCAN4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
Q8NAM6
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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