EFHD1 Antibody

ProSci
Product Code: PSI-5655
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5655-0.02mg0.02mg£150.00
Quantity:
PSI-5655-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of EFHD1 in human spleen tissue lysate with EFHD1 antibody at (A) 2 and (B) 4 μg/mL.
2 / 3
Immunofluorescence of EFHD1 in rat spleen tissue with EFHD1 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of EFHD1 in Rat Spleen cells with EFHD1 antibody at 20 μg/mL.

Western blot analysis of EFHD1 in human spleen tissue lysate with EFHD1 antibody at (A) 2 and (B) 4 μg/mL.
Immunofluorescence of EFHD1 in rat spleen tissue with EFHD1 antibody at 5 μg/mL.
Immunofluorescence of EFHD1 in Rat Spleen cells with EFHD1 antibody at 20 μg/mL.

Further Information

Additional Names:
EFHD1 Antibody: SWS2, MST133, PP3051, MSTP133, SWS2, EF-hand domain-containing protein D1, EF-hand domain-containing protein 1
Application Note:
EFHD1 antibody can be used for detection of EFHD1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunoflourescence starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
EFHD1 Antibody: EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.
Background References:
  • Tominaga M and Tomooka Y. Novel genes cloned from a neuronal cell line newly established from a cerebellum of an adult p53-/- mouse. Biochem. Biophys. Res. Comm.2002; 297:473-9.
Buffer:
EFHD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
EFHD1 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human EFHD1.

The immunogen is located within the first 50 amino acids of EFHD1.
NCBI Gene ID #:
80303
NCBI Official Name:
EF-hand domain family, member D1
NCBI Official Symbol:
EFHD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_079478
Protein GI Number:
20149496
Purification:
EFHD1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q9BUP0
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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