ZC3H12A Antibody

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ProSci
Product Code: PSI-5673
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5673-0.02mg0.02mg£150.00
Quantity:
PSI-5673-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of ZC3H12A in K562 cell lysate with ZC3H12A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Western blot analysis of ZC3H12A in K562 cell lysate with ZC3H12A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

Documents

Further Information

Additional Names:
ZC3H12A Antibody: MCPIP, MCPIP1, dJ423B22.1, MCPIP, Ribonuclease ZC3H12A, MCP-induced protein 1
Application Note:
ZC3H12A antibody can be used for detection of ZC3H12A by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
ZC3H12A Antibody: ZC3H12A, also known as MCPIP, is an essential member of a family of novel CCCH-zinc finger proteins that regulate macrophage activation and may be involved in host immunity and inflammatory diseases. ZC3H12A has RNase activity that prevents some immune disorders by directly affecting the mRNA stability of interleukins such as IL-6 and IL12p40. Mice lacking the ZC3H12A gene suffered from severe anemia, and most dies within 12 weeks. Overexpression of ZC3H12A causes ER stress and induces a number of genes involved in apoptosis and autophagy, including JNK, PUMA, and beclin-1 in cardiac myoblasts, leading to cell death. ZC3H12A can also induce adipogenesis in 3T3-L1 pre-adipocytes in the absence of PPAPgamma, demonstrating the complex roles ZC3H12A plays.
Background References:
  • Zhou L, Azfer A, Niu J, et al. Monocyte chemoattractant protein-1 induces a novel transcription factor that causes cardiac myocyte apoptosis and ventricular dysfunction. Circ. Res. 2006; 98:1177-85.
  • Liang J, Wang J, Azfer A, et al. A novel CCCH-zinc finger protein family regulates proinflammatory activation of macrophages. J. Biol. Chem. 2008; 283:6337-46.
  • Matsushita K, Takeuchi O, Standley DM, et al. Zc3h12a is an RNase essential for controlling immune responses by regulating mRNA decay. Nature 2009; 458:1185-90.
  • Younce CW and Kolattukudy PE. MCP-1 causes cardiomyoblast death via autophagy resulting from ER stress caused by oxidative stress generated by inducing a novel zinc-finger protein, MCPIP. Biochem. J. 2010; 426:43-53.
Buffer:
ZC3H12A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (82%)
Immunogen:
ZC3H12A antibody was raised against a 17 amino acid synthetic peptide near the center of human ZC3H12A.

The immunogen is located within amino acids 330 - 380 of ZC3H12A.
NCBI Gene ID #:
80149
NCBI Official Name:
zinc finger CCCH-type containing 12A
NCBI Official Symbol:
ZC3H12A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 66 kDa

Observed: 6 kDa
Protein Accession #:
NP_079355
Protein GI Number:
156151383
Purification:
ZC3H12A Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
SPECIFICITY:
This ZC3H12A antibody is predicted to have no cross-reactivity to other members of the ZC3H12 protein family.
Swissprot #:
Q5D1E8
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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