RANBP10 Antibody
| Code | Size | Price |
|---|
| PSI-5665-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5665-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
RANBP10 Antibody: KIAA1464, Ran-binding protein 10, RanBP10
Application Note:
RANBP10 antibody can be used for detection of RANBP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
RANBP10 Antibody: The RAN binding protein 10 (RANBP10) is a cytoplasmic guanine nucleotide exchange factor that shares high sequence similarity to the MET-interacting protein RANBP9. RANBP10 is expressed in the cytoplasm of mature megakaryocytes (MKs) and platelets where it localizes to polymerized noncentrosomal microtubules. RANBP10 possesses guanine nucleotide exchange factor activity towards RAN, a GTPase involved in nucleocytoplasmic traffic, spindle formation at mitosis, and nuclear envelope assembly during telophase, suggesting that RANBP10 may also be involved in these activities. RANBP10 depletion in vitro caused the disturbance of polymerized filaments in MKs and platelets in RANBP10-null mice exhibited disorders in microtubule filament numbers and localization, indicating that RANBP10 may play a significant role in maintaining microtubule dynamics.
Background References:
- Wang D, Zaibo Li, Schoen SR, et al. A novel MET-interacting protein shares high sequence similarity with RanBPM, but fails to stimulate MET-induced Ras/ERK signaling. Biochem. Biophys. Res. Comm.2004; 313:320-6.
- Schulze H, Dose M, Korpal M, et al. RanBP10 is a cytoplasmic guanine nucleotide exchange factor that modulates noncentrosomal microtubules. J. Biol. Chem.2008; 283:14109-19.
- Dasso M. The Ran GTPase: theme and variations. Curr. Biol.2002; R502-8.
- Kunert S, Meyer I, Fleischhauer S, et al. The microtubule modulator RanBP10 plays a critical role in regulation of platelet discoid shape and degranulation. Blood2009; 114:5532-40.
Buffer:
RANBP10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%), Mouse: (87%)
Immunogen:
RANBP10 antibody was raised against a 15 amino acid synthetic peptide near the center of human RANBP10.
The immunogen is located within amino acids 380 - 430 of RANBP10.
The immunogen is located within amino acids 380 - 430 of RANBP10.
NCBI Gene ID #:
57610
NCBI Official Name:
RAN binding protein 10
NCBI Official Symbol:
RANBP10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
EAW83167
Protein GI Number:
119603573
Purification:
RANBP10 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q6VN20
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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