ENC-2 Antibody

ProSci
Product Code: PSI-5695
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5695-0.02mg0.02mg£150.00
Quantity:
PSI-5695-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Chicken
Antibody Isotype: IgY
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of ENC-2 in Jurkat cell lysate with ENC-2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 3
Immunocytochemistry of ENC-2 in Jurkat cells with ENC-2 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of ENC-2 in Jurkat cells with ENC-2 antibody at 20 μg/mL.

Western blot analysis of ENC-2 in Jurkat cell lysate with ENC-2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of ENC-2 in Jurkat cells with ENC-2 antibody at 5 μg/mL.
Immunofluorescence of ENC-2 in Jurkat cells with ENC-2 antibody at 20 μg/mL.

Further Information

Additional Names:
ENC-2 Antibody: ENC2, ENC-2, ENC2, Kelch-like protein 25, Ectoderm-neural cortex protein 2
Application Note:
ENC-2 antibody can be used for detection of ENC-2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ENC-2 Antibody: The ectoderm-neural cortex-2 (ENC-2) protein a member of the BTB/PDZ Kelch protein family displaying significant homology to ENC-1, an actin-binding protein involved in neural fate specification during embryogenesis and adipocyte maturation. The exact function of ENC-2 has yet to be determined, but it has been suggested to be involved in the differentiation of neuronal precursor cells into neurons and astrocytes.
Background References:
  • Hernandez MC, Andres-Barquin PJ, Martinez S, et al. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. J. Neurosci.1997; 17:3038-51.
  • Zhao L, Gregoire F and Sul HS. Transient induction of ENC-1, a Kelch-related actin-binding protein, is required for adipocyte differentiation. J. Biol. Chem.2000; 275:16845-50.
  • Ahn JI, Kim SY, Ko MJ, et al. Analysis of gene expression in mouse spinal cord-derived neural precursor cells during neuronal differentiation. Gen. and Infor.2009; 7:85-96.
Buffer:
ENC-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ENC-2 antibody was raised against a 14 amino acid synthetic peptide near the center of human ENC-2.

The immunogen is located within amino acids 230 - 280 of ENC-2.
NCBI Gene ID #:
64410
NCBI Official Name:
kelch-like 25 (Drosophila)
NCBI Official Symbol:
KLHL25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAV51405
Protein GI Number:
55418570
Purification:
ENC-2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q9H0H3
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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