APC3 Antibody
| Code | Size | Price |
|---|
| PSI-5723-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5723-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
APC3 Antibody: APC3, HNUC, NUC2, ANAPC3, CDC27Hs, D0S1430E, D17S978E, Cell division cycle protein 27 homolog, Anaphase-promoting complex subunit 3, APC3
Application Note:
APC3 antibody can be used for detection of APC3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
APC3 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC3, also known as CDC27, a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), is a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. APC3 contains a tetratricopeptide repeat (TPR) region and interacts with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1.
Background References:
- JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
- Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
Buffer:
APC3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
APC3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human APC3.
The immunogen is located within the last 50 amino acids of APC3.
The immunogen is located within the last 50 amino acids of APC3.
NCBI Gene ID #:
996
NCBI Official Name:
cell division cycle 27 homolog (S. cerevisiae)
NCBI Official Symbol:
CDC27
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001247
Protein GI Number:
167466175
Purification:
APC3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
P30260
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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