APC2 Antibody

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ProSci
Product Code: PSI-5721
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5721-0.02mg0.02mg£150.00
Quantity:
PSI-5721-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of APC2 in HeLa cell lysate with APC2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of APC2 in HeLa cells with APC2 antibody at 5 μg/mL.
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Immunofluorescence of APC2 in HeLa cells with APC2 antibody at 20 μg/mL.
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Western blot analysis of APC2 in HeLa cell lysate with APC2 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of APC2 in HeLa cells with APC2 antibody at 5 μg/mL.
Immunofluorescence of APC2 in HeLa cells with APC2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
APC2 Antibody: APCL, APCL, Adenomatous polyposis coli protein 2, Adenomatous polyposis coli protein-like, APC-like
Application Note:
APC2 antibody can be used for detection of APC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
APC2 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC2 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. Like APC11, APC2 contains cullin and RING finger domains that are thought to be important in regulating ubiquitination activity.
Background References:
  • JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
  • Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
  • Yu H, Peters JM, King RW, et al. Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science1998; 279:1219-22.
  • Zacharie W, Shevchenko A, Andrews PD, et al. Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science1998; 1216-19.
Buffer:
APC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
APC2 antibody was raised against an 18 amino acid synthetic peptide near the center of human APC2.

The immunogen is located within amino acids 240 - 290 of APC2.
NCBI Gene ID #:
10297
NCBI Official Name:
adenomatosis polyposis coli 2
NCBI Official Symbol:
APC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
BAA34611
Protein GI Number:
3894265
Purification:
APC2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
O95996
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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