APC4 Antibody
| Code | Size | Price |
|---|
| PSI-5725-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5725-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
APC4 Antibody: APC4, APC4, Anaphase-promoting complex subunit 4, Cyclosome subunit 4
Application Note:
APC4 antibody can be used for detection of APC4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
APC4 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC4 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC4, it is thought that APC4 associates with other APC/C components APC1, APC5, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.
Background References:
- JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
- Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
- Thronton BR, Ng TM, Matyskiela ME, et al. An architectural map of the anaphase-promoting complex. Genes Dev.2006; 20:449-60.
Buffer:
APC4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
APC4 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human APC4.
The immunogen is located within amino acids 40 - 90 of APC4.
The immunogen is located within amino acids 40 - 90 of APC4.
NCBI Gene ID #:
29945
NCBI Official Name:
anaphase promoting complex subunit 4
NCBI Official Symbol:
ANAPC4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9UJX5
Protein GI Number:
205371737
Purification:
APC4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q9UJX5
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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