APC5 Antibody

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ProSci
Product Code: PSI-5727
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5727-0.02mg0.02mg£150.00
Quantity:
PSI-5727-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of APC5 in human kidney tissue lysate with APC5 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of APC5 in rat kidney tissue with APC5 antibody at 5 μg/mL.
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Immunofluorescence of APC5 in rat kidney tissue with APC5 antibody at 20 μg/mL.
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Western blot analysis of APC5 in human kidney tissue lysate with APC5 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of APC5 in rat kidney tissue with APC5 antibody at 5 μg/mL.
Immunofluorescence of APC5 in rat kidney tissue with APC5 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
APC5 Antibody: APC5, APC5, Anaphase-promoting complex subunit 5, Cyclosome subunit 5
Application Note:
APC5 antibody can be used for detection of APC5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
APC5 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC5, it is thought that APC5 associates with other APC/C components APC1, APC4, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.
Background References:
  • JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
  • Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
  • Thronton BR, Ng TM, Matyskiela ME, et al. An architectural map of the anaphase-promoting complex. Genes Dev.2006; 20:449-60.
Buffer:
APC5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%)
Immunogen:
APC5 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC5.

The immunogen is located within amino acids 480 - 530 of APC5.
NCBI Gene ID #:
51433
NCBI Official Name:
anaphase promoting complex subunit 5
NCBI Official Symbol:
ANAPC5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9UJX4
Protein GI Number:
37537861
Purification:
APC5 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q9UJX4
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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