APC6 Antibody

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ProSci
Product Code: PSI-5729
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5729-0.02mg0.02mg£150.00
Quantity:
PSI-5729-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of APC6 in human liver tissue lysate with APC6 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of APC6 in human liver tissue with APC6 antibody at 5 μg/mL.
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Immunofluorescence of APC6 in human liver tissue with APC6 antibody at 20 μg/mL.
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Western blot analysis of APC6 in human liver tissue lysate with APC6 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of APC6 in human liver tissue with APC6 antibody at 5 μg/mL.
Immunofluorescence of APC6 in human liver tissue with APC6 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
APC6 Antibody: APC6, CUT9, ANAPC6, Cell division cycle protein 16 homolog, Anaphase-promoting complex subunit 6, APC6
Application Note:
APC6 antibody can be used for detection of APC6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
APC6 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC6 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC6, it has been shown to interact with and is stabilized by CDC26 through an intermolecular TPR mimic composed of one helix from each protein.
Background References:
  • JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
  • Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
  • Wang J, Dye BT, Rajashankar KR, et al. Insights into anaphase promoting complex TPR subdomain assembly from a CDC26-APC structure. Nat. Struct. Mol. Biol.2009; 16:987-9.
Buffer:
APC6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
APC6 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human APC6.

The immunogen is located within the first 50 amino acids of APC6.
NCBI Gene ID #:
8881
NCBI Official Name:
cell division cycle 16 homolog (S. cerevisiae)
NCBI Official Symbol:
CDC16
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003894
Protein GI Number:
118402580
Purification:
APC6 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q13042
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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