APC7 Antibody
| Code | Size | Price |
|---|
| PSI-5731-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5731-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
APC7 Antibody: APC7, APC7, Anaphase-promoting complex subunit 7, Cyclosome subunit 7
Application Note:
APC7 antibody can be used for detection of APC7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
APC7 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC7 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC7 is required for proper protein ubiquitination function of APC/C and for the interaction of APC/C with various transcription coactivators.
Background References:
- JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
- Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
Buffer:
APC7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
APC7 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human APC7.
The immunogen is located within amino acids 520 - 570 of APC7.
The immunogen is located within amino acids 520 - 570 of APC7.
NCBI Gene ID #:
51434
NCBI Official Name:
anaphase promoting complex subunit 7
NCBI Official Symbol:
ANAPC7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 66 kDa
Observed: 68 kDa
Observed: 68 kDa
Protein Accession #:
NP_057322
Protein GI Number:
212549736
Purification:
APC7 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q9UJX3
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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