APC8 Antibody

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ProSci
Product Code: PSI-5733
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5733-0.02mg0.02mg£150.00
Quantity:
PSI-5733-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of APC8 in K562 cell lysate with APC8 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of APC8 in K562 cells with APC8 antibody at 5 μg/mL.
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Western blot analysis of APC8 in K562 cell lysate with APC8 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of APC8 in K562 cells with APC8 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
APC8 Antibody: APC8, CUT23, ANAPC8, Cell division cycle protein 23 homolog, Anaphase-promoting complex subunit 8, APC8
Application Note:
APC8 antibody can be used for detection of APC8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
APC8 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC8 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. In Drosophila, silencing of APC8 results in developmental delay and pupal lethality with elevated levels of apoptosis, high mitotic index, and delayed or blocked mitosis.
Background References:
  • JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
  • Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
  • Pal M, Nagy O, Menesi D, et al. Structurally related TPR subunits contribute differently to the function of the anaphase-promoting complex in Drosophila melanogaster. J. Cell Sci.2007; 120:3238-48.
Buffer:
APC8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
APC8 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC8.

The immunogen is located within amino acids 270 - 320 of APC8.
NCBI Gene ID #:
8697
NCBI Official Name:
cell division cycle 23 homolog (S. cerevisiae)
NCBI Official Symbol:
CDC23
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9UJX2
Protein GI Number:
254763423
Purification:
APC8 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q9UJX2
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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