PIAS1 Antibody
| Code | Size | Price |
|---|
| PSI-5741-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5741-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PIAS1 Antibody: GBP, ZMIZ3, DDXBP1, GU/RH-II, E3 SUMO-protein ligase PIAS1, Gu-binding protein, GBP
Application Note:
PIAS1 antibody can be used for detection of PIAS1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
PIAS1 Antibody: The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated alpha-globin expression in erythroid cells.
Background References:
- Liu B, Liao J, Rao X, et al. Inhibition of Stat1-mediated gene activation by PIAS1. Proc. Natl. Acad. Sci. USA1998; 95: 10626-31.
- Shuai K and Liu B. Regulation of gene-activation pathways by PIAS proteins in the immune system. Nat. Rev. Immunol.2005; 5:593-605.
- Kang HC, Chae JH, Jeon J, et al. PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific {alpha}-globin expression. Nuc. Acids Res.2010 Apr 26.
Buffer:
PIAS1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PIAS1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human PIAS1.
The immunogen is located within the last 50 amino acids of PIAS1.
The immunogen is located within the last 50 amino acids of PIAS1.
NCBI Gene ID #:
8554
NCBI Official Name:
protein inhibitor of activated STAT, 1
NCBI Official Symbol:
PIAS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057250
Protein GI Number:
7706637
Purification:
PIAS1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
O75925
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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