PIAS3 Antibody
| Code | Size | Price |
|---|
| PSI-5745-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5745-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PIAS3 Antibody: ZMIZ5, E3 SUMO-protein ligase PIAS3, Protein inhibitor of activated STAT protein 3
Application Note:
PIAS3 antibody can be used for detection of PIAS3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
PIAS3 Antibody: The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS3. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS3 binds specifically to STAT3 following the stimulation of STAT3. Increased expression of PIAS3 has been observed in several human cancers, including lung, breast, and brain tumors, but not in anaplastic lymphoma kinase-positive T/null-cell lymphomas, indicating that PIAS3 plays multiple roles in different tissue and cell types.
Background References:
- Shuai K and Liu B. Regulation of gene-activation pathways by PIAS proteins in the immune system. Nat. Rev. Immunol.2005; 5:593-605.
- Chung CD, Liao J, Liu B, et al. Specific inhibition of Stat3 signal transduction by PIAS3. Science1997; 278:1803-5.
- Wang L and Banerjee S. Differential PIAS3 expression in human malignancy. Oncol. Rep.2004; 11:1319-24.
- Zhang Q, Raghunath PN, Xue L, et al. Multilevel dysregulation of STAT3 activation in anaplastic lymphoma kinase-positive T/null-cell lymphoma. J. Immunol.2002; 168:466-7
Buffer:
PIAS3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PIAS3 antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human PIAS3.
The immunogen is located within amino acids 410 - 460 of PIAS3.
The immunogen is located within amino acids 410 - 460 of PIAS3.
NCBI Gene ID #:
10401
NCBI Official Name:
protein inhibitor of activated STAT, 3
NCBI Official Symbol:
PIAS3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006090
Protein GI Number:
115298686
Purification:
PIAS3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q9Y6X2
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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