PIAS4 Antibody

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ProSci
Product Code: PSI-5747
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5747-0.02mg0.02mg£150.00
Quantity:
PSI-5747-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of PIAS4 in HL60 cell lysate with PIAS4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 2
Immunohistochemistry of PIAS4 in rat brain tissue with PIAS4 antibody at 5 μg/mL.
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Western blot analysis of PIAS4 in HL60 cell lysate with PIAS4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PIAS4 in rat brain tissue with PIAS4 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
PIAS4 Antibody: PIASY, Piasg, ZMIZ6, PIASG, E3 SUMO-protein ligase PIAS4, PIASy, PIAS-gamma
Application Note:
PIAS4 antibody can be used for detection of PIAS4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
PIAS4 Antibody: The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS4. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS4 interacts with p53 and blocks its ability to induce Bax and p21 transcription. PIAS4 is also important in the control of the ubiquitin-dependent proteasomal degradation of the Ets-1 transcription factor. PIAS4 has been implicated in the DNA-damage response pathway and is thought to work in combination with PIAS1 for the productive association of 53BP1, BRCA1 and RNF168.
Background References:
  • Shuai K and Liu B. Regulation of gene-activation pathways by PIAS proteins in the immune system. Nat. Rev. Immunol. 2005; 5:593-605.
  • Nelson V, Davis GE, and Maxwell SA. A putative protein inhibitor of activated STAT (PIASy) interacts with p53 and inhibits p53-,ediated transactivation but not apoptosis. Apoptosis 2001; 6:221-34.
  • Nishida T, Terashima M, Fukami K, et al. PIASy controls ubiquitin-dependent proteasomal degradation of Ets-1. Biochem. J. 2007; 405:481-8.
  • Galanty Y, Belotserkovskaya R, Coates J, et al. Mammalian SUMO E3-ligases PIAS1 and PIAS4 promote responses to DNA double-strand breaks. Nature 2009; 462:935-9.
Buffer:
PIAS4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PIAS4 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human PIAS4.

The immunogen is located within amino acids 50 - 100 of PIAS4.
NCBI Gene ID #:
51588
NCBI Official Name:
protein inhibitor of activated STAT, 4
NCBI Official Symbol:
PIAS4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 56 kDa

Observed: 56 kDa
Protein Accession #:
NP_056981
Protein GI Number:
24850133
Purification:
PIAS4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q8N2W9
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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